pegfp c1 ar v7  (Addgene inc)

Journal: Cellular & Molecular Biology Letters

Article Title: Inhibition of the RORC/GPX4 mediated ferroptosis regulatory axis suppresses tumor growth and alleviates enzalutamide resistance in prostate cancer

doi: 10.1186/s11658-025-00846-z

Figure Lengend Snippet: Ferroptosis agonist reverses enzalutamide resistance induced by high RORC expression. A . Normal C4-2B cells morphology and enzalutamide half inhibition rate. B . The half inhibition rate and cells morphology of enzalutamide on oe RORC /C4-2B cells. C . The culture of enzalutamide-resistant C4-2B cells (C4-2B/ENZR). D . The half inhibition rate and cells morphology of enzalutamide-resistant C4-2B cells (C4-2B/ENZR). E . The expression of RORC in C4-2B/ENZR cells was detected by western blot, C4-2B/ENZR vs C4-2B: *** P -value ≤ 0.001. F . Assessment of the inhibitory effect of enzalutamide (ENZ) on C4-2B cell proliferation. G . Evaluation of the inhibitory efficacy of erastin (ERA) on the proliferative capacity of C4-2B cells. H . Cell counting telomere fluorescence assay was used to detect the proliferation ability of oe RORC /C4-2B cells. I . The effect of oe RORC /C4-2B cells proliferation was detected by plate cloning assay. J . Western blot was used to detect the expression of apoptosis and proliferation proteins. K . Western blot was used to detect the expression of AR, AR-V7, and ROR-γ in oe RORC /C4-2B cells. L . Western blot was used to detect the expression of ferroptosis protein. M . Cell counting assay was used to detect the proliferation ability of oe RORC /C4-2B cells. N . Visualization of plate cloning experiment histogram. O . Protein expression quantification of apoptosis and proliferation markers. P . Quantification of AR, AR-V7, and ROR-γ protein expression levels. Q . Quantification of ferroptosis protein expression levels. R . GSH content in oe RORC /C4-2B cells. S. MDA content in oe RORC /C4-2B cells. T . Fe 2+ content in oe RORC /C4-2B cells. U . ROS fluorescence experiment histogram visualization. V . ROS fluorescence assay was used to detect the content of ROS in oe RORC /C4-2B cells. W . JC-1 assay was used to detect the changes of mitochondrial membrane potential in oe RORC /C4-2B cells. X . JC-1 experiment histogram visualization. * P -value ≤ 0.1; ** P -value ≤ 0.01; *** P -value ≤ 0.001; **** P -value ≤ 0.0001. NS no significance

Article Snippet: Experimental materials are listed below: N-cadherin (66219–1-Ig, Proteintech), vimentin (E-AB-18212, Proteintech), Snail (26183–1-AP, Proteintech), MMP2 (E-AB-40409, Proteintech), Parp-1 (13371–1-AP, Proteintech), AR (22089–1-AP, Proteintech), AR-V7 (68492S, Cell Signaling Technology), RORC (PA5-23148, Thermo Scientific), E-cadherin (20874–1-AP, Proteintech), c-Myc (10828–1-AP, Proteintech), cyclin D1 (26939–1-AP, Proteintech), cleaved caspase 7 (9491S, Cell Signaling Technology), GPX4 (67763–1-Ig, Proteintech), SLC7A11 (26864–1-AP, Proteintech), FTH1 (10727–1-AP, Proteintech), and GAPDH (60004–1-Ig, Proteintech).

Techniques: Expressing, Inhibition, Western Blot, Cell Counting, Fluorescence, Cloning, Membrane

Journal: Cellular & Molecular Biology Letters

Article Title: Inhibition of the RORC/GPX4 mediated ferroptosis regulatory axis suppresses tumor growth and alleviates enzalutamide resistance in prostate cancer

doi: 10.1186/s11658-025-00846-z

Figure Lengend Snippet: Ilicicolin A inhibited the proliferation of CRPC cells through induction of ferroptosis. A . Expression levels of AR, AR-V7, and ROR-γ were detected by western blot. B . Western blot assay was used to evaluate the effect of ilicicolin A on ferroptosis-related proteins. C . Measurement of MDA content in 22Rv1 cells. D . Measurement of MDA content in C4-2B cells. E . Measurement of GSH content in 22Rv1 cells. F . Measurement of GSH content in C4-2B cells. G . Measurement of Fe 2+ content in 22Rv1 cells. H . Measurement of Fe 2+ content in C4-2B cells. I . Fe 2+ levels in CRPC cells were assessed using the ferroOrange fluorescence assay. J . Histogram visualization of the ferroOrange fluorescence experiment. * P -value ≤ 0.1; ** P -value ≤ 0.01; *** P -value ≤ 0.001; **** P -value ≤ 0.0001. NS no significance

Article Snippet: Experimental materials are listed below: N-cadherin (66219–1-Ig, Proteintech), vimentin (E-AB-18212, Proteintech), Snail (26183–1-AP, Proteintech), MMP2 (E-AB-40409, Proteintech), Parp-1 (13371–1-AP, Proteintech), AR (22089–1-AP, Proteintech), AR-V7 (68492S, Cell Signaling Technology), RORC (PA5-23148, Thermo Scientific), E-cadherin (20874–1-AP, Proteintech), c-Myc (10828–1-AP, Proteintech), cyclin D1 (26939–1-AP, Proteintech), cleaved caspase 7 (9491S, Cell Signaling Technology), GPX4 (67763–1-Ig, Proteintech), SLC7A11 (26864–1-AP, Proteintech), FTH1 (10727–1-AP, Proteintech), and GAPDH (60004–1-Ig, Proteintech).

Techniques: Expressing, Western Blot, Fluorescence

Journal: Cellular & Molecular Biology Letters

Article Title: Inhibition of the RORC/GPX4 mediated ferroptosis regulatory axis suppresses tumor growth and alleviates enzalutamide resistance in prostate cancer

doi: 10.1186/s11658-025-00846-z

Figure Lengend Snippet: Ilicicolin A suppresses tumor growth in vivo. A . General information of in vivo experiments on the detection of CRPC proliferation ability by ilicicolin A. B . Effect of ilicicolin A on body weight of nude mice. C . The size of tumor volume in nude mice was measured and recorded in vivo. D . The results of subcutaneous tumor formation in nude mice. E . The heart, lung, spleen, liver, and kidney were observed by HE staining. F . Immunohistochemistry was used to detect the expression of ferroptosis antibody, AR, AR-V7, and ROR-γ. G . Western blot was used to detect the expression of apoptosis and proliferation proteins. H . Western blot was used to detect the expression of AR, AR-V7, and ROR-γ. I . Western blot was used to detect the expression of ferroptosis protein. * P -value ≤ 0.1; ** P -value ≤ 0.01; *** P -value ≤ 0.001; **** P -value ≤ 0.0001. NS no significance

Article Snippet: Experimental materials are listed below: N-cadherin (66219–1-Ig, Proteintech), vimentin (E-AB-18212, Proteintech), Snail (26183–1-AP, Proteintech), MMP2 (E-AB-40409, Proteintech), Parp-1 (13371–1-AP, Proteintech), AR (22089–1-AP, Proteintech), AR-V7 (68492S, Cell Signaling Technology), RORC (PA5-23148, Thermo Scientific), E-cadherin (20874–1-AP, Proteintech), c-Myc (10828–1-AP, Proteintech), cyclin D1 (26939–1-AP, Proteintech), cleaved caspase 7 (9491S, Cell Signaling Technology), GPX4 (67763–1-Ig, Proteintech), SLC7A11 (26864–1-AP, Proteintech), FTH1 (10727–1-AP, Proteintech), and GAPDH (60004–1-Ig, Proteintech).

Techniques: In Vivo, Staining, Immunohistochemistry, Expressing, Western Blot

Journal: Cellular & Molecular Biology Letters

Article Title: Inhibition of the RORC/GPX4 mediated ferroptosis regulatory axis suppresses tumor growth and alleviates enzalutamide resistance in prostate cancer

doi: 10.1186/s11658-025-00846-z

Figure Lengend Snippet: Ilicicolin A induces ferroptosis and regresses tumor growth of CRPC in vivo. A . General information on the in vivo experiment of ilicicolin A enhancing the sensitivity of CRPC to enzalutamide. B . Effects of ilicicolin A and Enzalutamide on body weight of nude mice. C . The size of tumor volume in nude mice was measured and recorded in vivo by ilicicolin A and enzalutamide. D . The results of subcutaneous tumor formation in nude mice. E . The heart, lung, spleen, liver, and kidney were observed by HE staining experimental. F . Immunohistochemistry was used to detect the expression of ferroptosis antibody, AR, AR-V7, and ROR-γ. G . Western blot was used to detect the expression level of apoptosis and proliferation proteins. H . Western blot was used to detect the expression level of AR, AR-V7, and ROR-γ. I . Western blot was used to detect the expression of ferroptosis protein. * P -value ≤ 0.1; ** P -value ≤ 0.01; *** P -value ≤ 0.001; **** P -value ≤ 0.0001. NS no significance

Article Snippet: Experimental materials are listed below: N-cadherin (66219–1-Ig, Proteintech), vimentin (E-AB-18212, Proteintech), Snail (26183–1-AP, Proteintech), MMP2 (E-AB-40409, Proteintech), Parp-1 (13371–1-AP, Proteintech), AR (22089–1-AP, Proteintech), AR-V7 (68492S, Cell Signaling Technology), RORC (PA5-23148, Thermo Scientific), E-cadherin (20874–1-AP, Proteintech), c-Myc (10828–1-AP, Proteintech), cyclin D1 (26939–1-AP, Proteintech), cleaved caspase 7 (9491S, Cell Signaling Technology), GPX4 (67763–1-Ig, Proteintech), SLC7A11 (26864–1-AP, Proteintech), FTH1 (10727–1-AP, Proteintech), and GAPDH (60004–1-Ig, Proteintech).

Techniques: In Vivo, Staining, Immunohistochemistry, Expressing, Western Blot

Journal: Cellular & Molecular Biology Letters

Article Title: Inhibition of the RORC/GPX4 mediated ferroptosis regulatory axis suppresses tumor growth and alleviates enzalutamide resistance in prostate cancer

doi: 10.1186/s11658-025-00846-z

Figure Lengend Snippet: Ilicicolin A alleviates enzalutamide resistance by RORC/GPX4 axis of CRPC in vivo. A . General information on the in vivo experiment of ilicicolin A enhancing the sensitivity of CRPC to enzalutamide. B . Effects of ilicicolin A, enzalutamide, and erastin on body weight of nude mice. C . In vivo measurement and recording of the tumor volume of ilicicolin A, enzalutamide, and erastin in nude mice. D . The results of subcutaneous tumor formation in nude mice. E . The heart, lung, spleen, liver, and kidney were observed by HE staining. F . Immunohistochemistry was used to detect the expression of ferroptosis antibody, AR, AR-V7, and ROR-γ. G . Western blot was used to detect the expression of apoptosis and proliferation proteins. H . Western blot was used to detect the expression of AR, AR-V7, and ROR-γ. I . Western blot was used to detect the expression of ferroptosis protein. * P -value ≤ 0.1; ** P -value ≤ 0.01; *** P -value ≤ 0.001; **** P -value ≤ 0.0001. NS no significance

Article Snippet: Experimental materials are listed below: N-cadherin (66219–1-Ig, Proteintech), vimentin (E-AB-18212, Proteintech), Snail (26183–1-AP, Proteintech), MMP2 (E-AB-40409, Proteintech), Parp-1 (13371–1-AP, Proteintech), AR (22089–1-AP, Proteintech), AR-V7 (68492S, Cell Signaling Technology), RORC (PA5-23148, Thermo Scientific), E-cadherin (20874–1-AP, Proteintech), c-Myc (10828–1-AP, Proteintech), cyclin D1 (26939–1-AP, Proteintech), cleaved caspase 7 (9491S, Cell Signaling Technology), GPX4 (67763–1-Ig, Proteintech), SLC7A11 (26864–1-AP, Proteintech), FTH1 (10727–1-AP, Proteintech), and GAPDH (60004–1-Ig, Proteintech).

Techniques: In Vivo, Staining, Immunohistochemistry, Expressing, Western Blot